The SCIF is able to assist in design of flow cytometry and FACS experiments. Users can have their samples run with operator assistance, or can run their samples independently with the proper training. We also recommend using FluoroFinder for flow cytometry panel design. Please click here to visit our UC Merced-specific FluoroFinder page. Below are the SCIF instruments and their specifications:
Bio-Rad ZE5 - Flow cytometer
5 laser system: 355nm UV, 405nm Violet, 488nm Blue, 561nm Yellow, 640nm Red
Designed for high throughput ananlysis of up to 27 color panels
Automated tube and plate loading ability up to 384-well plates
Small particle detection
Instrument configuration and fluorochrome guide
21-color panel example voltages and compensation matrix
BD LSR II - Flow cytometer
4 laser system: 355nm, 405nm, 488nm, 633nm
Designed for high throughput ananlysis of up to 11 color panels
Instrument configuration and fluorochrome guide
BD FACS Aria III - Flow cytometer/Cell Sorter with Aerosol Management
4 laser system: 405nm, 488nm, 561nm, 633nm
561 laser allows maximal detection of dsRed and mCherry
Aerosol Management System is required for BSL-2 applications (eg. human cells, infectious agents)
Instrument configuration and fluorochrome guide
BD FACS Aria II - Flow cytometer/Cell Sorter
3 laser system: 407nm, 488nm, 633nm
Instrument configuration and fluorochrome guide
Analysis Workstation
We have a workstation running FlowJo V10 located in SE153. The workstation is designed to be used for post-acquision analysis. It is free of use, saving you the expense of purchasing your own flow analysis software.
